It needs to be stored wrapped in foil and replaced every 2 months. Toxic to humans, use PPE! Rifampicin is TOXIC to humans, light-sensitive, and degrades in solution rather quickly. While dissolving Rifampicin in MeOH, there might be a small residue left at the bottom which may not go into solution even after adding a few drops of NaOH. Plates start to lose sensitivity after 2 weeks recommended Kanamycin plates only last a few weeks at 4☌ best to make fresh Kan plates every 2 - 4 weeks. Use 20 µg/mL in culture from 20mg/mL 1000× stock Selection strength may vary in non-model bacteria May crystallize when added to liquid culture. coli colonies on 8-day old MG-Dcs plates that had spent 3 of those days at -20☌ and 5 at 4☌, compared to what was found on 1 day old MG-Dcs. A single small-scale experiment found 6-10x as many E. D-cycloserine is best used within two days of pouring.
Carbenicillin is more stable than Ampicillin, and is generally preferable.ĭegrades noticeably over the course of 1 week at 4☌. In general, use carbenicillin for ampicillin selection, because it is more chemically stable.Ĭarbenicillin can be used in place of Ampicillin at the same working concentration. Stock solutions can be stored at 4☌ for 2 weeks but can last as long as 4-6 months when stored at -20☌. Culture plates with Amp can be stored at 4☌ for about 2 weeks. Antibiotic selection in other organisms may use different concentrations, so check primary literature for more info.Īmpicillin degrades quickly in both plates and stock solutions. This table lists “standard” concentrations of antibiotics commonly used in microbiology laboratories. Antibiotic concentrations and stock solutions
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